Objective To investigate the effect and mechanism of Avastin in corneal lymphangiogenesis and angiogenesis, we observed the existence of lymphatic vessels and blood vessels in mouse cornea in different stage after alkali burns. Methods The mouse models with alkali burned cornea were built by sodium hydroxide. The models were divided into control group and treatment group randomly. Treatment group were injected 25mg/ml Avastin 5?l subconjunctivally once every two days as well as levofloxacin three times a day for two weeks. Control group were given levofloxacin eyedrops three times a day for two weeks. Corneas were excised at the stages of 3d, 7d, and 12d after alkali burns respectively. The corneal lymphatic vessels were detected by lymphatic vessel endothelial hyaluronic acid receptor（LYVE-1） as the specific marker and the corneal neovascularization was evaluated under stereomicroscope. The expression of vascular endothelial growth factor C VEGF-C) was observed by immunohistochemical stain. Results Newly formed lymphatic vessels were showed in corneal stroma at the stage of 7d after alkali burns in control group, lymphatic vessels were observed at the stage of 12d in Avastin treatment group. The corneal neovascularization was decreased in Avastin injected group compared with that in control group. In normal cornea, epithelium and endothelium showed staining of VEGF-C protein. In Avastin treatment group, the expression of VEGF-C decreased at different stage comparing with corresponding control group. Conclusion Avastin decreases corneal lymphangiogenesis and angiogenesis in alkali burned cornea. The mechanism might inhibit the expression of VEGF-C in cornea after alkali burns