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Avastin对碱烧伤角膜内新生淋巴管和血管形成的影响
王进,孙亮,齐秀英
0
()
摘要:
目的 观察小鼠碱烧伤后角膜新生淋巴管和血管的形成情况,探讨Avastin对小鼠角膜新生淋巴管和血管形成的影响及其机制。 方法 制作小鼠角膜碱烧伤模型,随机分为治疗组和对照组,治疗组小鼠25mg/ml Avastin 5?l结膜下隔日注射,同时左氧氟沙星滴眼液滴眼3次/日,连续治疗两周;对照组连续两周内每日左氧氟沙星滴眼液滴眼3次,分别于碱烧伤后3d、7d和12d 取材。应用LYVE-1标记淋巴管,观察小鼠角膜内新生淋巴管的形成情况;体视显微镜观察角膜新生血管形成情况。免疫组化法检测血管生成因子VEGF-C在角膜碱烧伤后不同时间段角膜组织内的表达。 结果 碱烧伤后7d的对照组角膜基质中可见新生淋巴管。在治疗组中,碱烧伤后12d可见角膜基质内存在新生的淋巴管。Avastin治疗组不同时间段角膜新生血管的形成均少于角膜碱烧伤对照组。在正常小鼠角膜组织内,VEGF-C表达于角膜上皮层和内皮层。在治疗组碱烧伤角膜内,各时间段VEGF-C的表达均低于对照组。 结论 Avastin具有抑制小鼠角膜碱烧伤后角膜新生淋巴管和新生血管形成的作用,其机制可能是通过抑制角膜组织内VEGF-C的表达从而抑制角膜新生淋巴管和新生血管的形成。
关键词:  血管内皮生长因子C  淋巴管  血管  角膜碱烧伤
DOI:0
基金项目:
The Effect of Avastin on Lymphangiogenesis and Angiogenesis in Cornea after Alkali Burns
()
Abstract:
Objective To investigate the effect and mechanism of Avastin in corneal lymphangiogenesis and angiogenesis, we observed the existence of lymphatic vessels and blood vessels in mouse cornea in different stage after alkali burns. Methods The mouse models with alkali burned cornea were built by sodium hydroxide. The models were divided into control group and treatment group randomly. Treatment group were injected 25mg/ml Avastin 5?l subconjunctivally once every two days as well as levofloxacin three times a day for two weeks. Control group were given levofloxacin eyedrops three times a day for two weeks. Corneas were excised at the stages of 3d, 7d, and 12d after alkali burns respectively. The corneal lymphatic vessels were detected by lymphatic vessel endothelial hyaluronic acid receptor(LYVE-1) as the specific marker and the corneal neovascularization was evaluated under stereomicroscope. The expression of vascular endothelial growth factor C VEGF-C) was observed by immunohistochemical stain. Results Newly formed lymphatic vessels were showed in corneal stroma at the stage of 7d after alkali burns in control group, lymphatic vessels were observed at the stage of 12d in Avastin treatment group. The corneal neovascularization was decreased in Avastin injected group compared with that in control group. In normal cornea, epithelium and endothelium showed staining of VEGF-C protein. In Avastin treatment group, the expression of VEGF-C decreased at different stage comparing with corresponding control group. Conclusion Avastin decreases corneal lymphangiogenesis and angiogenesis in alkali burned cornea. The mechanism might inhibit the expression of VEGF-C in cornea after alkali burns
Key words:  VEGF-C  Lymphatic vessel  Blood vessel  Corneal alkali burns

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